Not known Details About thr77
Not known Details About thr77
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two extra splice variants were being documented but detailed nucleotide sequence information and facts is not yet out there.
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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide normally incorporates the epitope regarded from the antibody.
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This guideline outlines even further possible optimization of mobile and immunoassay parameters to be certain the absolute best outcomes are...
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This guidebook outlines even further possible optimization of cellular and immunoassay parameters to make certain the absolute best effects are...
Antibodies sure to the blocking peptide now not bind to the epitope on the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody by itself, you can see which staining is specific.
Focus on Information and facts Cyclin E belongs to your really conserved cyclin loved ones, whose customers are characterized by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which add towards the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities as a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates at the G1-S stage boundary and it is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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